Review



antibodies against insulin  (Developmental Studies Hybridoma Bank)


Bioz Verified Symbol Developmental Studies Hybridoma Bank is a verified supplier
Bioz Manufacturer Symbol Developmental Studies Hybridoma Bank manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Developmental Studies Hybridoma Bank antibodies against insulin
    Antibodies Against Insulin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/anti-insulin/bio_rxiv__64898__2026__04__25__720819-298-9-14
    Average 94 stars, based on 127 article reviews
    antibodies against insulin - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Clinical-grade cryopreservation unlocks transplant-ready human pancreatic and stem cell–derived islets for diabetes therapy
    Article Snippet: .. Slides were incubated overnight at 4 °C with primary antibodies against insulin (Cat. #GN-ID4, DSHB) and glucagon (Cat. #G2654-100UL, Sigma-Aldrich). ..



    Similar Products

    antibodies against insulin  (Developmental Studies Hybridoma Bank)
    94
    Developmental Studies Hybridoma Bank antibodies against insulin
    Antibodies Against Insulin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/anti-insulin/bio_rxiv__64898__2026__04__25__720819-298-9-14
    Average 94 stars, based on 1 article reviews
    antibodies against insulin - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    rabbit polyclonal antibody against insulin  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc rabbit polyclonal antibody against insulin
    Rabbit Polyclonal Antibody Against Insulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/Insulin+Antibody/pm41891636-86-0-11
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal antibody against insulin - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    antibodies against insulin  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc antibodies against insulin
    Antibodies Against Insulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/Insulin+Rabbit+mAb/pmc12887434-170-12-16
    Average 95 stars, based on 1 article reviews
    antibodies against insulin - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    antibody against insulin  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc antibody against insulin
    Antibody Against Insulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/Insulin+Antibody/pm41548912-54-6-11
    Average 96 stars, based on 1 article reviews
    antibody against insulin - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    antibodies against insulin like growth factor 1 igf 1  (Affinity Biosciences)
    86
    Affinity Biosciences antibodies against insulin like growth factor 1 igf 1
    Antibodies Against Insulin Like Growth Factor 1 Igf 1, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/15+anti+factor+klf15+kruppel+like/pm41430614-132-22-30
    Average 86 stars, based on 1 article reviews
    antibodies against insulin like growth factor 1 igf 1 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    monoclonal antibodies against phospho insulin receptor β  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology monoclonal antibodies against phospho insulin receptor β
    Effects of Pas2r12 or Pas2r12–cargo protein complex-mediated stimulation on <t>INSR/IGF1R</t> and ERK1/2. HEK293 cells were pretreated with dimethyl sulfoxide (linsitinib [−]) or with linsitinib (linsitinib [+]) and subsequently stimulated with Pas2r12, Pas2r12–EGFP, Pas2r12–IgG, or insulin for 2 min ( A – C ) or 10 min ( D – F ). Panels A and D show representative Western blot images. Panels B and E display the phosphorylation levels of INSR/IGF1R (pINSR/pIGF1R); panels C and F show the phosphorylation levels of ERK1/2 (pERK1/2); all values were normalized to GAPDH. Phosphorylation levels were analyzed and compared with the solvent control (DMEM only, linsitinib [−]) using Student’s t -test. For each treatment condition, linsitinib (+) was compared with the corresponding linsitinib (−) condition. Statistically significant differences compared with the control are indicated on the bar graphs; significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Statistical comparisons were performed against control cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01. N = 4.
    Monoclonal Antibodies Against Phospho Insulin Receptor β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/p-insulin+R%CE%B2+Antibody/pmc12736253-192-5-15
    Average 93 stars, based on 1 article reviews
    monoclonal antibodies against phospho insulin receptor β - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    antibodies against insulin  (R&D Systems)
    93
    R&D Systems antibodies against insulin
    Effects of Pas2r12 or Pas2r12–cargo protein complex-mediated stimulation on <t>INSR/IGF1R</t> and ERK1/2. HEK293 cells were pretreated with dimethyl sulfoxide (linsitinib [−]) or with linsitinib (linsitinib [+]) and subsequently stimulated with Pas2r12, Pas2r12–EGFP, Pas2r12–IgG, or insulin for 2 min ( A – C ) or 10 min ( D – F ). Panels A and D show representative Western blot images. Panels B and E display the phosphorylation levels of INSR/IGF1R (pINSR/pIGF1R); panels C and F show the phosphorylation levels of ERK1/2 (pERK1/2); all values were normalized to GAPDH. Phosphorylation levels were analyzed and compared with the solvent control (DMEM only, linsitinib [−]) using Student’s t -test. For each treatment condition, linsitinib (+) was compared with the corresponding linsitinib (−) condition. Statistically significant differences compared with the control are indicated on the bar graphs; significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Statistical comparisons were performed against control cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01. N = 4.
    Antibodies Against Insulin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+insulin/Human%2FMouse%2FBovine+Insulin+Antibody/pm41353923-56-9-15
    Average 93 stars, based on 1 article reviews
    antibodies against insulin - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of Pas2r12 or Pas2r12–cargo protein complex-mediated stimulation on INSR/IGF1R and ERK1/2. HEK293 cells were pretreated with dimethyl sulfoxide (linsitinib [−]) or with linsitinib (linsitinib [+]) and subsequently stimulated with Pas2r12, Pas2r12–EGFP, Pas2r12–IgG, or insulin for 2 min ( A – C ) or 10 min ( D – F ). Panels A and D show representative Western blot images. Panels B and E display the phosphorylation levels of INSR/IGF1R (pINSR/pIGF1R); panels C and F show the phosphorylation levels of ERK1/2 (pERK1/2); all values were normalized to GAPDH. Phosphorylation levels were analyzed and compared with the solvent control (DMEM only, linsitinib [−]) using Student’s t -test. For each treatment condition, linsitinib (+) was compared with the corresponding linsitinib (−) condition. Statistically significant differences compared with the control are indicated on the bar graphs; significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Statistical comparisons were performed against control cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01. N = 4.

    Journal: Pharmaceuticals

    Article Title: Role of the Insulin Receptor in Mediating Cytosolic Delivery of Proteins by a Modified Cell-Penetrating Peptide

    doi: 10.3390/ph18121885

    Figure Lengend Snippet: Effects of Pas2r12 or Pas2r12–cargo protein complex-mediated stimulation on INSR/IGF1R and ERK1/2. HEK293 cells were pretreated with dimethyl sulfoxide (linsitinib [−]) or with linsitinib (linsitinib [+]) and subsequently stimulated with Pas2r12, Pas2r12–EGFP, Pas2r12–IgG, or insulin for 2 min ( A – C ) or 10 min ( D – F ). Panels A and D show representative Western blot images. Panels B and E display the phosphorylation levels of INSR/IGF1R (pINSR/pIGF1R); panels C and F show the phosphorylation levels of ERK1/2 (pERK1/2); all values were normalized to GAPDH. Phosphorylation levels were analyzed and compared with the solvent control (DMEM only, linsitinib [−]) using Student’s t -test. For each treatment condition, linsitinib (+) was compared with the corresponding linsitinib (−) condition. Statistically significant differences compared with the control are indicated on the bar graphs; significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Statistical comparisons were performed against control cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01. N = 4.

    Article Snippet: Membranes were incubated with primary monoclonal antibodies against phospho-insulin receptor β (p-IRβ; clone 10C3, sc-81500; Santa Cruz Biotechnology, Dallas, TX, USA), INSR (MAA895Hu21; Cloud-Clone Corp., Houston, TX, USA), phospho-ERK1/2 (sc-7383; Santa Cruz Biotechnology), ERK1/2 (sc-514302; Santa Cruz Biotechnology), and IGF-1 receptor (MAB659Hu22; Cloud-Clone Corp).

    Techniques: Western Blot, Phospho-proteomics, Solvent, Control

    Effect of IGF1R and INSR knockdown on the Pas2r12-mediated cytosolic delivery of EGFP. Western blot analyses ( A – C ) and confocal laser scanning microscopy images ( D , E ). Graphs B and C show IGF1R and INSR expression levels, respectively, normalized to GAPDH. Cells analyzed with confocal laser scanning microscopy in panel A were analyzed by Western blot ( A – C ). ( D ) Pas2r12-mediated cytosolic delivery of EGFP in knockdown cells, with EGFP fluorescence shown in green, and ( E ) percentage of cells exhibiting cytosolic EGFP delivery. Scale bars represent 20 μm. Statistical comparisons were performed against siNC cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05, *** p < 0.001. N = 4.

    Journal: Pharmaceuticals

    Article Title: Role of the Insulin Receptor in Mediating Cytosolic Delivery of Proteins by a Modified Cell-Penetrating Peptide

    doi: 10.3390/ph18121885

    Figure Lengend Snippet: Effect of IGF1R and INSR knockdown on the Pas2r12-mediated cytosolic delivery of EGFP. Western blot analyses ( A – C ) and confocal laser scanning microscopy images ( D , E ). Graphs B and C show IGF1R and INSR expression levels, respectively, normalized to GAPDH. Cells analyzed with confocal laser scanning microscopy in panel A were analyzed by Western blot ( A – C ). ( D ) Pas2r12-mediated cytosolic delivery of EGFP in knockdown cells, with EGFP fluorescence shown in green, and ( E ) percentage of cells exhibiting cytosolic EGFP delivery. Scale bars represent 20 μm. Statistical comparisons were performed against siNC cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05, *** p < 0.001. N = 4.

    Article Snippet: Membranes were incubated with primary monoclonal antibodies against phospho-insulin receptor β (p-IRβ; clone 10C3, sc-81500; Santa Cruz Biotechnology, Dallas, TX, USA), INSR (MAA895Hu21; Cloud-Clone Corp., Houston, TX, USA), phospho-ERK1/2 (sc-7383; Santa Cruz Biotechnology), ERK1/2 (sc-514302; Santa Cruz Biotechnology), and IGF-1 receptor (MAB659Hu22; Cloud-Clone Corp).

    Techniques: Knockdown, Western Blot, Confocal Laser Scanning Microscopy, Expressing, Fluorescence

    Effects of Pas2r12 or Pas2r12–cargo protein complex stimulation on INSR/IGF1R in IGF1R-overexpressing cells. ( A ) HEKI#66 cells pretreated with DMSO (linsitinib [−]) or linsitinib (linsitinib [+]) were stimulated with Pas2r12, Pas2r12–EGFP, or insulin for 2 min. Level of phosphorylated INSR/IGF1R (pINSR/pIGF1R) was assessed by Western blot. ( B ) Relative levels of pINSR/pIGF1R, normalized to GAPDH, corresponding to the data in panel A. Phosphorylation levels were analyzed using Student’s t -test. Each treatment condition was compared with the solvent-treated HEKI#66 control (linsitinib [−]), and, for each treatment, phosphorylation levels in the linsitinib (−) and linsitinib (+) conditions were also compared. Statistically significant differences compared with the solvent-treated control are indicated on the bar graphs, and significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Error bars indicate the standard error of the mean (SEM). * p < 0.05, *** p < 0.001. N = 3.

    Journal: Pharmaceuticals

    Article Title: Role of the Insulin Receptor in Mediating Cytosolic Delivery of Proteins by a Modified Cell-Penetrating Peptide

    doi: 10.3390/ph18121885

    Figure Lengend Snippet: Effects of Pas2r12 or Pas2r12–cargo protein complex stimulation on INSR/IGF1R in IGF1R-overexpressing cells. ( A ) HEKI#66 cells pretreated with DMSO (linsitinib [−]) or linsitinib (linsitinib [+]) were stimulated with Pas2r12, Pas2r12–EGFP, or insulin for 2 min. Level of phosphorylated INSR/IGF1R (pINSR/pIGF1R) was assessed by Western blot. ( B ) Relative levels of pINSR/pIGF1R, normalized to GAPDH, corresponding to the data in panel A. Phosphorylation levels were analyzed using Student’s t -test. Each treatment condition was compared with the solvent-treated HEKI#66 control (linsitinib [−]), and, for each treatment, phosphorylation levels in the linsitinib (−) and linsitinib (+) conditions were also compared. Statistically significant differences compared with the solvent-treated control are indicated on the bar graphs, and significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Error bars indicate the standard error of the mean (SEM). * p < 0.05, *** p < 0.001. N = 3.

    Article Snippet: Membranes were incubated with primary monoclonal antibodies against phospho-insulin receptor β (p-IRβ; clone 10C3, sc-81500; Santa Cruz Biotechnology, Dallas, TX, USA), INSR (MAA895Hu21; Cloud-Clone Corp., Houston, TX, USA), phospho-ERK1/2 (sc-7383; Santa Cruz Biotechnology), ERK1/2 (sc-514302; Santa Cruz Biotechnology), and IGF-1 receptor (MAB659Hu22; Cloud-Clone Corp).

    Techniques: Western Blot, Phospho-proteomics, Solvent, Control

    Assessment of INSR overexpression. ( A ) Verification of INSR expression levels in INSR-overexpressing (IN) cells. ( B ) Relative levels of INSR expression normalized to GAPDH, based on the data in panel A. The value for INSR/GAPDH in parental HEK293 cells was set to 1.0 for comparison. Statistical comparisons were performed against HEK293 cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01. N = 3. ( C ) Subcellular localization of INSR in IN cells. Green indicates INSR, and blue indicates nuclei. Scale bars represent 20 μm.

    Journal: Pharmaceuticals

    Article Title: Role of the Insulin Receptor in Mediating Cytosolic Delivery of Proteins by a Modified Cell-Penetrating Peptide

    doi: 10.3390/ph18121885

    Figure Lengend Snippet: Assessment of INSR overexpression. ( A ) Verification of INSR expression levels in INSR-overexpressing (IN) cells. ( B ) Relative levels of INSR expression normalized to GAPDH, based on the data in panel A. The value for INSR/GAPDH in parental HEK293 cells was set to 1.0 for comparison. Statistical comparisons were performed against HEK293 cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01. N = 3. ( C ) Subcellular localization of INSR in IN cells. Green indicates INSR, and blue indicates nuclei. Scale bars represent 20 μm.

    Article Snippet: Membranes were incubated with primary monoclonal antibodies against phospho-insulin receptor β (p-IRβ; clone 10C3, sc-81500; Santa Cruz Biotechnology, Dallas, TX, USA), INSR (MAA895Hu21; Cloud-Clone Corp., Houston, TX, USA), phospho-ERK1/2 (sc-7383; Santa Cruz Biotechnology), ERK1/2 (sc-514302; Santa Cruz Biotechnology), and IGF-1 receptor (MAB659Hu22; Cloud-Clone Corp).

    Techniques: Over Expression, Expressing, Comparison

    Effects of Pas2r12 or Pas2r12–cargo protein complex stimulation on INSR/IGF1R in INSR-overexpressing cells. ( A ) IN#1 cells pretreated with DMSO (linsitinib [−]) or linsitinib (linsitinib [+]) were stimulated with Pas2r12, Pas2r12–EGFP, or insulin for 2 min. Phosphorylation of INSR/IGF1R (pINSR/pIGF1R) was assessed by Western blot. ( B ) Relative levels of pINSR/pIGF1R were normalized to GAPDH, corresponding to the data in panel A. Phosphorylation levels were analyzed using Student’s t -test. Each treatment condition was compared with the solvent-treated IN#1 control (linsitinib [−]), and for each treatment, phosphorylation levels in the linsitinib (−) and linsitinib (+) conditions were also compared. Statistically significant differences compared with the solvent-treated control are indicated on the bar graphs, and significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Error bars indicate the standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001. N = 3.

    Journal: Pharmaceuticals

    Article Title: Role of the Insulin Receptor in Mediating Cytosolic Delivery of Proteins by a Modified Cell-Penetrating Peptide

    doi: 10.3390/ph18121885

    Figure Lengend Snippet: Effects of Pas2r12 or Pas2r12–cargo protein complex stimulation on INSR/IGF1R in INSR-overexpressing cells. ( A ) IN#1 cells pretreated with DMSO (linsitinib [−]) or linsitinib (linsitinib [+]) were stimulated with Pas2r12, Pas2r12–EGFP, or insulin for 2 min. Phosphorylation of INSR/IGF1R (pINSR/pIGF1R) was assessed by Western blot. ( B ) Relative levels of pINSR/pIGF1R were normalized to GAPDH, corresponding to the data in panel A. Phosphorylation levels were analyzed using Student’s t -test. Each treatment condition was compared with the solvent-treated IN#1 control (linsitinib [−]), and for each treatment, phosphorylation levels in the linsitinib (−) and linsitinib (+) conditions were also compared. Statistically significant differences compared with the solvent-treated control are indicated on the bar graphs, and significant differences between linsitinib (+) and linsitinib (−) treatments are marked by horizontal lines. Error bars indicate the standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001. N = 3.

    Article Snippet: Membranes were incubated with primary monoclonal antibodies against phospho-insulin receptor β (p-IRβ; clone 10C3, sc-81500; Santa Cruz Biotechnology, Dallas, TX, USA), INSR (MAA895Hu21; Cloud-Clone Corp., Houston, TX, USA), phospho-ERK1/2 (sc-7383; Santa Cruz Biotechnology), ERK1/2 (sc-514302; Santa Cruz Biotechnology), and IGF-1 receptor (MAB659Hu22; Cloud-Clone Corp).

    Techniques: Phospho-proteomics, Western Blot, Solvent, Control

    Effect of INSR overexpression on the Pas2r12-mediated cytosolic delivery of EGFP. ( A ) Representative confocal images showing cellular uptake of Pas2r12–EGFP in INSR-overexpressing (IN) cells. Merged images show EGFP fluorescence (green), Hoechst 33342 nuclear staining (blue), and differential interference contrast. Scale bars represent 20 μm. ( B ) Relative levels of the cytosolic delivery efficiency of EGFP in IN cells compared with parental HEK293 cells (set to 100%). Statistical comparisons were performed against HEK293 cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05. N = 3.

    Journal: Pharmaceuticals

    Article Title: Role of the Insulin Receptor in Mediating Cytosolic Delivery of Proteins by a Modified Cell-Penetrating Peptide

    doi: 10.3390/ph18121885

    Figure Lengend Snippet: Effect of INSR overexpression on the Pas2r12-mediated cytosolic delivery of EGFP. ( A ) Representative confocal images showing cellular uptake of Pas2r12–EGFP in INSR-overexpressing (IN) cells. Merged images show EGFP fluorescence (green), Hoechst 33342 nuclear staining (blue), and differential interference contrast. Scale bars represent 20 μm. ( B ) Relative levels of the cytosolic delivery efficiency of EGFP in IN cells compared with parental HEK293 cells (set to 100%). Statistical comparisons were performed against HEK293 cells using Student’s t -test. Error bars indicate the standard error of the mean (SEM). * p < 0.05. N = 3.

    Article Snippet: Membranes were incubated with primary monoclonal antibodies against phospho-insulin receptor β (p-IRβ; clone 10C3, sc-81500; Santa Cruz Biotechnology, Dallas, TX, USA), INSR (MAA895Hu21; Cloud-Clone Corp., Houston, TX, USA), phospho-ERK1/2 (sc-7383; Santa Cruz Biotechnology), ERK1/2 (sc-514302; Santa Cruz Biotechnology), and IGF-1 receptor (MAB659Hu22; Cloud-Clone Corp).

    Techniques: Over Expression, Fluorescence, Staining